Normal penetration of rabbit spermatozoa through a trypsin- and acrosin-resistant zona pellucida
نویسندگان
چکیده
منابع مشابه
The Passage of Spermatozoa through and into the Zona Pellucida of the Rabbit Egg.
Polyspermy is pathological in mammals (Austin & Walton, i960), but is uncommon because mechanisms exist which prevent its occurrence. In the sheep, cow, pig and other species, after penetration by the first spermatozoon, the zona pellucida undergoes a change, the 'zona reaction', which bars further spermatozoa from passing through the zona, but still allows them to enter it. Unlike the eggs of ...
متن کاملSperm penetration through cumulus mass and zona pellucida.
Mammalian fertilization requires sperm to penetrate the cumulus mass and egg zona pellucida prior to fusion with the egg. Although sperm penetration through these physical barriers is essential, the molecular mechanism has not yet been completely elucidated. In addition to sperm motility, hyaluronan-hydrolyzing and proteolytic enzymes of sperm have been suggested to participate in the penetrati...
متن کاملProtease activity involvement in the passage of mammalian sperm through the zona pellucida.
The interaction between acrosome-reacted sperm and zona pellucida proteins is not yet fully understood. Serine protease acrosin and its zymogen proacrosin have been proposed to fulfill this function due to their capacity to bind zona pellucida glycoproteins. However, the molecular mechanism underlying this interaction has been merely speculative. Here we show that fucoidan (a sulfated polysacch...
متن کاملSperm Penetration of the Zona Pellucida of the Pig Egg.
In most mammals that have been studied, the first spermatozoon which penetrates an egg stimulates the egg to undergo a reaction—the 'zona reaction'—which bars further spermatozoa from penetrating through the zona pellucida (Braden, Austin & David, 1954). The sequel to the zona reaction may be classified into two categories, according to species: species in which spermatozoa are entirely prevent...
متن کاملPurification of mouse sperm acrosin, its activation from proacrosin and effect on homologous egg investments.
When proacrosin from mouse epididymal spermatozoa was activated a single form of acrosin was produced. The enzyme was isolated by gel filtration followed by affinity chromatography using Sepharose-4B linked to an acrosin inhibitor p-(p'-aminophenoxypropoxy)benzamidine. The molecular weight of partly purified acrosin was 53 000 by gel filtration, and of the pure enzyme 39 000 by SDS-polyacrylami...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction
سال: 1978
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0540385